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KMID : 0363620170380040001
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Journal of Korean Oriental Medicine
2017 Volume.38 No. 4 p.1 ~ p.10
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The effects of Pongamia pinnata on osteogenic differentiation and mineralization of human stem cells derived from the gingiva
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Lee Hyun-Jin
Uddin Md. Salah
Kim Yong-In
Park Jun-Beom
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Abstract
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Objectives: The aim of the present study is to evaluate the effects of the extract of Pongamia pinnata on the morphology, viability, and differentiation potential of human stem cells derived from the gingiva.
Methods: Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of methanol extract of Pongamia pinnata (PPT) at concentrations ranging from 0.001 to 1%. Evaluations of cell morphology and cellular viability were done at Day 1. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells.
Results: The morphology of stem cells in the presence of PPT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not produce any noticeable changes when compared with the untreated control group. Application of PPT produced a significant increase in alkaline phosphatase activity when compared to the control group. The results of the Alizarin Red S staining showed a significant increase of absorbance with the 0.001% group.
Conclusions: Based on these findings, it was concluded that PPT could produce beneficial effects on mesenchymal stem cells with enhanced osteogenic differentiation.
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KEYWORD
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cell differentiation, herbal medicine, Pongamia, stem cells
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